ATCC 100 Test Method for Antibacterial Finishes on Textile Materials: Assess

Purpose of Test

The test method aims to quantify the antibacterial properties of textile materials.

This test method is designed to evaluate various types of textiles by performing a 24-hour contact assessment of the bacteriostatic (growth inhibition) and bactericidal (bacteria elimination) properties.

Terminology Defined

Antibacterial Activity – Measures the protection a textile provides against microorganisms, biological fluids, and aerosols.

Bacteriostatic Properties – The capacity of the textile to prevent bacteria growth.

Bactericidal Properties – The capacity of textile to kill bacteria.

Nutritive Broth – A neutralizing solution that helps microorganisms to grow during the test when diluted.

Test Method

Materials Used for Test

  • Untreated sample of textile
  • Sterile nutritive solution
  • Petri dish
  • Sealed jars

Testing Procedure

The test undergoes 6 stages to complete the evaluation

  1. Preparation of samples
  2. Sterilization
  3. Inoculation
  4. Incubation
  5. Washing or shaking out
  6. Counting

Test Procedure (Summary)

  • Part 1: To start the test, a microorganism is cultured in liquid form. It is standardized to learn the concentration levels of the test microorganism.
  • Part 2: The microbial culture is diluted through the use of a sterile nutritive solution.
  • Part 3: The control and test fabric samples are inoculated with microorganisms. The microbial suspension touches just the sample textile.
  • Part 4: The bacteria levels of the control and test fabrics are identified as “time zero” when washed out with a nutritive broth, then proceeded with dilution and plating.
  • Part 5: To verify the effectiveness of the neutralization of antimicrobial agent in the textile, a control is implemented.
  • Part 6: Additional inoculations are done to test the control and test fabrics. The materials are left to incubate in sealed jars for 24 hours.
  • Part 7: Once the incubation is completed, it is time to identify the microbial concentrations. The decrease in the number of microorganisms from the initial concentrations and control fabric is measured.

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